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/FontFamily (Arial) /FontName /Arial /FontStretch /Normal /FontWeight 400 /ItalicAngle 0 /StemV 88 /Type /FontDescriptor /XHeight 519 >> endobj 148 0 obj << /Author (Shalini Sharma; Dr. Rajeshwari Shivaraj; Dr. A. Radhakrishna) /CreationDate (D:20140530173758+05'30') /Creator (International Journal of Scientific Engineering and Research \(IJSER\), www.ijser.in) /Keywords (Melamine; Milk; LC-MS/MS; Sand cleanup; Polar.\r\n) /ModDate (D:20201103154654+05'30') /Producer (Total PDF Watermark Remover 1.0.214) /Subject (A simple, precise, accurate and validated Liquid chromatography and Tandem mass spectrometric \(LC-MS/MS\) method is developed for the analysis of melamine in whole milk. Because of melamines basic and polar nature, a cation exchange natural sand material is used as cleanup technique. As a result, enhancement in recovery, sensitivity and selectivity has been observed as well made the analysis much cheaper by replacement of use of cartridge. A simple extraction of melamine with phosphate buffer \(in acidic condition\) and Acetonitrile \(precipitation of the protein\), matrix cleanup using silica based strong cationic exchangers \(sand\) and reconstitution with 0.1% Formic acid \(v/v\) in Acetonitrile. The separation is performed on a Inertsil ODS-3C18 column \(150mm x 4.6mm x 3m. Two selected reaction monitoring \(SRM\) transitions are monitored for Melamine. After optimization of instrument parameter and settings, the linear response \(r2>0.999\) is observed for samples ranging from 0.50 to 10 g/kg. The method provides recoveries of 70-120% in the concentration range of 0.50 to 10 g/kg, intra- and inter-day variation in <2.5% RSD. The limit of detection \(LOD\) and limit of quantification \(LOQ\) values are 0.2 and 0.6 g/kg, respectively with an uncertainty of 0.03g/kg at 95 % confidence level.\r\n) /Title (The Novel Method of Extraction and Analysis of Melamine in Whole Milk Using Sand Material Cleanup Technique and Inertsil C18 Column by LC-MS/MS) >> endobj 161 0 obj << /Ascent 1026 /CapHeight 662 /Descent -307 /Flags 34 /FontBBox [ -558 -307 2000 1026 ] /FontFamily (Times New Roman) /FontFile2 181 0 R /FontName /ZLAINQ+TimesNewRomanPS-BoldMT /FontStretch /Normal /FontWeight 700 /ItalicAngle 0 /StemV 136 /Type /FontDescriptor /XHeight 457 >> endobj 10 0 obj << /AIS false /BM /Normal /CA 1 /OP false /OPM 1 /SA false /SMask /None /Type /ExtGState /ca 1 /op false >> endobj 184 0 obj [ /ICCBased 180 0 R ] endobj 187 0 obj << /Ascent 1032 /CapHeight 662 /Descent -307 /Flags 98 /FontBBox [ -547 -307 1401 1032 ] /FontFamily (Times New Roman) /FontFile2 186 0 R /FontName /IJOKJY+TimesNewRomanPS-BoldItalicMT /FontStretch /Normal /FontWeight 700 /ItalicAngle -17 /StemV 124 /Type /FontDescriptor /XHeight 453 >> endobj 1 0 obj << /Contents 2 0 R /CropBox [ 0 0 595.22 842 ] /MediaBox [ 0 0 595.22 842 ] /Parent 194 0 R /Resources << /ColorSpace << /CS0 157 0 R /CS1 158 0 R /CS2 158 0 R >> /ExtGState << /GS0 160 0 R /GS1 173 0 R >> /Font << /C2_0 138 0 R /TT0 155 0 R /TT1 156 0 R /TT2 169 0 R /TT3 172 0 R >> /ProcSet [ /PDF /Text /ImageC ] /XObject << /Im0 176 0 R /Im1 177 0 R >> >> /Rotate 0 /Type /Page >> endobj 2 0 obj << /Filter /FlateDecode /Length 7259 >> stream HWr8~-rKb8ެS&f@S MeE}$[jJ%h F_N7) Ig0i٬N^YQ#8Pς0L8N)_Z`6$T ,LI!"-zw!}WY%0,0H)M(/`sAY-VӸSv,WnTEDEYƴx߽k_y-kn\:ZNh">}r 29w+_ʵ'UI.Y&jVn̻]7mtWk?ͯ<+M hy.EٌMEqAYd@% eBʪV4sJ^ATP>+xt44Az_Z{M-{0OS/AJ)HqcT5;)v %YnY:pjXo[tt3[ BMա5X_!{M*awQG3R75KP 8H~=>zu9<*7iA+ARFK|dؘn웵cgRu]/ug7'fY=Tڝlȯf6ƪRh˵>% >oYE