Analysis of Amylase Inhibitors Content and Characterizations of Cassava Adira - 1 Variety: (Manihot esculenta Crantz) as a Functional Food

Eduan Effendi, Basuni Hamzah, Agus Wijaya, Taufik Indrajaya, Rindit Pambayun, Husniyati Bastari

Abstract: The results of activity test analysis of amylase inhibitors early stages obtained 16 samples amylase inhibitors extracted from cassava varieties of Adira-1 (Manihot esculenta crantz) by using the ?-enzyme amylase of marine animals Brevibacterium sp. Based on the analysis, that the sample number 2, 3, 4, 6, 10, 11, 13, 14 and 15 had a positive value, it means predictable that to 9 samples amylase inhibitors that have the ability to inhibit ?-amylase. Based on the protein content test in amylase inhibitors using the method of Lowry, that the protein value of 16 samples ranged from 0.325 mg / mL to 0.588 mg / mL. After purification the result that the molecular weight amylase inhibitors ranged from 20 kD to 113 kD. In this study, acquired 100% inhibition of the activity of amylase inhibitors against the action of the ?-amylase enzyme in the samples of cassava were experiencing treatment dredged mucus layer cassava and experience of immersion in water for 12 hours (P1L2) occurs at a volume of amylase inhibitor 400 ng, a temperature of 30 ? C, the concentration of 1% starch, ?-amylase enzyme volume 0.4 mg / mL and pH 6.7. Based on these results, amylase inhibitors of cassava (Manihot esculenta Crantz) had a potential inhibitory action of the ?-amylase enzyme, so that it could be made as a functional food which could be used for prevention, treatment of obesity and diabetes mellitus due to the isolation, purification and characterizations of specific amylase inhibitors in casava varieties of Adira-1 (Manihot esculenta Crantz) had made it known. For further research on the type of protein in cassava of amylase inhibitors with Immunobloting method or Western Bloting.

Keywords: Amylase inhibitors, inhibition, cassava, ?-amylase enzyme and Functional Food