Molecular Detection of Toxoplasma gondii in Native Freshwater Fish Cyprinuscarpio in Wasit Province Iraq

Altamemy A. K. Aakool, Shiemaa Jabbar Abidali

Abstract: DNA of total 96 samples of (24) muscle, (24) liver, (24) enteric and (24) gills of native freshwater fish Cyprinus carpio was extracted by Genomic DNA purification kit and tested using Real time polymerase chain reaction and specific set of primers and probe to detect B1 gene of Toxoplasma gondii. Sixteen enteric samples and only two gills samples was positive whereas the others was negative, this results refers to presence of T. gondii in this species of fish, a based to these results the infection may be acquired either from contaminated environment or due to consuming filter concentration invertebrates. The negative results of muscle and liver may be due to localization of parasites in organs that close contact with environment and the parasites either have no ability to diffuse to visceral organs or have no capable to survive in these organs.

Keywords: toxoplasma , fish , iraq , real time , PCR