Cloning Expression of Arsenic Binding Protein with GFP Tag in Bl21de3 Strain and its Chracterization

Avan Lal, Somnath

Abstract: This review explores the cloning, expression, and of arsenic binding protein bind with green fluorescent protein (GFP) tags in Escherichia coli BL21DE3 strain. Arsenic contamination poses significant environmental and health risks, necessitating the study of proteins involved in arsenic detoxification and sequestration. GFP tagging facilitates visualization and tracking of these proteins, aiding in their characterization and potential applications. The cloning process involves selecting suitable expression vectors and host strains, followed by DNA manipulation and vector construction. Expression optimization parameters, including inducer con - centration, temperature, and culture media, play crucial roles in achieving high protein yields. Characterization techniques such chromatography and SDS - PAGE, and fluorescence microscopy allow for the assessment of protein folding, stability, and arsenic binding affinity. Studies have implications for biosensing, bioremediation, and biomedical research. Future directions include structural elucidation and protein engineering to enhance the efficiency and specificity of arsenic binding proteins.

Keywords: Arsenic binding protein (ABP), GFP tag, BL21 (DE3), Arsenic biosensor, Protein expression and purification